CMMB 511 Lecture Notes - Lecture 15: Half-Life, Escherichia Coli, Cytokine
28 Dec 2019
School
Course
Professor
Document Summary
All mature transcripts end up in the cytoplasm for functioning (protein synthesis) Mrnas stay in cytoplasm for varied amounts of time depending on its stability. Stability is another level of control of gene control. Difficult to measure mrna stability because transcription goes on all the time, so there are transcripts coming in from nucleus, but at the same time mrnas are degraded at varying rates. The balance between transcripts coming in and degradation determines stability. If you have a probe to measure the stability by hybridization. There are sequences in 3"utr of mrna that are very conserved and are involved in making mrnas stable or unstable. Cultured cells (human or rodents): 30 min or less (histone and cytokine mrnas), 3-24 hrs (specific mrnas of cultured cells), average half life is 10 hours. Histones are not polyadenylated, therefore shorter half life. As generation time increases (complexity of genome), half life increases.
