CMMB 545 Lecture Notes - Lecture 21: Syntrophy, Amplicon, Microbiological Culture
20 Jun 2020
School
Course
Professor
Document Summary
Electron donors are the cause for reservoir souring (ie. h2s production) which requires an electron donor. Certain hcs can act as donors, or fermentation products (ie. volatile fatty acids, or weak acids), hydrogen, or acetate. Both systems: parallel in what terminal members can do. Difference: methanogens cannot metabolize larger compounds, so they need electron donors that are broken down. If you have 13c labelled substrate, those carbon atoms will be incorporated into biomolecules (ie. dna and rna) Sip combines in situ tools in an ultimately enrichment technique because its extract dna or. Extracting genetic information from samples to provide snapshot of microbial community. Activity in nature is low or slow. In situ: apply molecular biology methods directly to sample and extracting biological molecules for further analysis. Amplicon sequencing typically involves 16s rrna genes, but can also be other functional genes such as dsra genes.
