BIOC 3560 Lecture Notes - Lecture 4: Intimate Ion Pair, Valine, Protonation

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29 Sep 2016

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Only leu at f4 and his at f8 are conserved in mb, alpha, and beta. In different globins, segments of mostly different amino acids form similar structures. Out of 150 amino acids, only 27 (18%) are identical between mb and hb subunits. What types of interactions hold the subunits of haemoglobin together: hydrophobic interactions, hydrogen bonds. 30 residues are involved in the interface between alpha 1-beta 1 and between alpha 2 beta 2 (these are the strongest interactions) 19 residues are involved i the interfaces between alpha 2- beta 1 and between alpha 1 - beta 2. Ion pairs (salt bridges) stabilize the conformation of hb. Several important pairs occur at the a1b2 and a2b1 interfaces. B1 subunit his hc3 (+) forms salt bridge with the b1 subunit at asp fg1 (-) and the a2 subunit at lys c5 (+) Salt bridges stabilize the t state, they are broken in the r state.

Related Questions

1. Which of the following pairings regarding protein structure is/are incorrect?

Question options: a) primary structure: alpha helix

b) quaternary structure: hydrophobic interactions between different regions of the same polypeptide strand

c) tertiary structure: interactions between R groups on different polypeptide chains

d) secondary structure: hydrogen bonding between R groups on the same polypeptide strand

e) All of the above are incorrect.

2. Given the following pairs of amino acids, which pair contains the correct type of interaction through which the two amino acids most likely interact?

Question options: a) valine and leucine: charge-charge (salt bridge)

b) threonine and cysteine: disulfide bond

c) aspartic acid and glutamic acid: hydrophobic interaction

d) threonine and serine: hydrogen bonding

e) None of the above is correct.

3. Which statement is correct regarding the comparison of the following three tripeptides:

1) lys-lys-lys

2) asp-asp-asp

3) lys-asp-val

Question options:

a) Under physiological conditions, peptide #1 elutes last using cation exchange chromatography.

b) Under physiological conditions, peptide #2 elutes first using anion exchange chromatography.

c) Under physiological conditions, peptide #3 elutes last using anion exchange chromatography.

d) Under physiological conditions, peptide #3 elutes first using either cation or anion exchange chromatography.

4. When varying the substrate concentration at a fixed concentration of enzyme it is observed that at low concentrations of substrate the reaction is ________ (with respect to substrate),while at high concentrations of substrate the reaction is ________ (with respect to substrate).

Question options: a) initial; maximal

b) maximal; initial

c) second order; first order

d) first order; zero order

BIOC 3560 Lecture Notes - Lecture 4: Intimate Ion Pair, Valine, Protonation

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