BIOL 1090 Lecture Notes - Lecture 30: Nuclear Pore, Nuclear Matrix, Nucleoplasm

• School: University of Guelph
• Department: Biology
• Course: BIOL 1090
• Professor: Jaideep Mathur

Document Summary

Storage, expression, replication and repair of genetic material ribosome biosynthesis. Structure: nuclear envelope and content (chromatin, nucleoplasm, nuclear matrix, nucleolus) Outer membrane: binds ribosomes and is continuous with rough er (rer) Inner membrane: integral membrane proteins that connect to nuclear lamina. Selective barrier: allows limiting movement of molecules between nucleus and cytoplasm. Thin meshwork of filaments: lamins (type of intermediate filament) ~ 10nm diameter, integrity regulated by phosphorylation. Bound to inner surface of nuclear envelope (ne) by: prenyl group linked to lamin polypeptide and integral membrane proteins. Progeria syndrome: mutation in lamina causing premature aging in children. Structural proteins: nuclear lamins and matrix, dna packing. Inner and outer membranes fuse at pores ~ 120nm diameter: gateways between cytoplasm and nucleoplasm, 3000-4000 pores per nucleus, facilitate nucleo-cytoplasmic transport. 100s of polypeptides: nucleoporins (nups, approx 30 diff nups. Fits into the pore; reduces functional diameter to approx. Fg-domains phenylalanine-glycine (fg) repeats in npc proteins help create a mesh.