BIOL 1090 Lecture Notes - Lecture 4: Golgi Apparatus, Endosymbiont, Cell Membrane
26 Feb 2018
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Origin of eukaryotic cells: the endosymbiont theory, lynn margulies ~ major proponent, certain organelles of the eukaryotic cell have evolved from smaller prokaryotic cells that have taken up residence within the cytoplasm of a larger host cell . ~ er, golgi, endosomes, lysosomes, and vacuoles together. Uses radioactive isotopes (r) and photographic emulsion: short incubation with r (pulse, incubation with non-r (chase) Varying the chase period located protein in different compartments and thus allows its movement to be followed over time. Gfp) fused to dna encoding protein to be studied (called fusion protein) Use of: introduced in a living cell, observed overtime. Cell homogenization: (breaking up) cells or isolating organelles - sub-cellurar fractionation. Cell free systems: do not contain whole cells (ex. discovery and characterization of ribosomes) A dynamic system comprising of interconnected membrane cisternae, tubules an vesicles enclosing a space (lumen) that is separated from the rest of the cell. Cytosol = aqueous compartment of cell consisting of organelles.
