BIOL 1090 Lecture Notes - Lecture 3: Photobleaching, Propidium Iodide, Covalent Bond
10 Mar 2016
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Document Summary
Depth of field: distance between nearest and farthest object that appear sharp in an image. Resolution: ability to resolve detail, distinguish between 2 points in image d=(0. 61)( )/nsin . Point sources of light from a specimin apear as airy disks. Limit of resolution of microscope is ability to distinguish between 2 closely spaced airy disks. Theoretical limit of resolution at given wavelength is rayleigh limit. Wavelength spectrum of light is major factor in degree of resolution. Shorter wavelengths resolve details better than long wavelengths. Numerical aperture is the collecting capacity of any lens system: nsin , in air = 1. High resolution due to wave properties of electrons. Electrons reflected on surface, bounce off specimin. Wavelength of electrons varies with their speeds changing according to accelerating voltage applied. Aromatic wings bonded so molecule can absorb part of visible spectrum. Metachromasy: dye stains tissue different colour from solution. Make structures more visible when not adequately highlighted by primary stain.
