MBG 3660 Lecture Notes - Lecture 21: Inert Gas, Arginine, High-Performance Liquid Chromatography

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Used to identify the role proteins play in biological processes, molecular function and cellular components. Yeast 2-hybrid can be used to identify interacting partners. Fused to the dna binding domain (dbd) of a transcription factor that activates the. His3 gene: no his3 transcription because no activation domain, orf fused to activation domain (ad) but lacks dbd. Prey: library of every open reading frame in genome: combine a) and b) and grow in the absence of histidine. Need b and orf to get histidine production and growth. Used to identify biological process and possibly some function. Information about biological process, function and cellular components. Proteome: the identity and quantity of every protein in a cell at a given time. This is done by using the proteins mass/charge ratio with techniques such as 2d gel separation (isoelectric point and molecular weight) Expasy proteomics server: used to compute the pi/mw. Doesn"t (cid:449)ork for (cid:271)asi(cid:272) proteins, small peptides and proteins difficult to solubilize.

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