MCB 2050 Lecture Notes - Lecture 6: Dna Microarray, Ecori, Ecotype

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: Ray Lu

Document Summary

Genomics is the subdiscipline of genetics devoted to mapping, sequencing, and functional and comparative analyses of genomces. Physical maps: an average frequency of recombination of 1% Genetic maps: often constructed using molecular markers such as restriction fragments or different lengths (rflps restriction fragment. Physical map distances to not correlate perfectly with genetic. There are many correlations between the different kinds of. Maps that are marked both physically and genetically are called. Restriction length polymorphism (rflp: mutations in nucleotide sequences in restriction enzyme cleavage sites result in the formation of new restriction sites of various lengths referred to as rflps. Page 1 of 11: some rflps can be separated with electrophoresis, stained with ethidium bromide, and viewed under uv light. Others can be detected using cdna as radioactive probes on genomic southern blots. In humans the most useful rflps are short tandem repeats called variable number tandem repeats (vntrs, or microsatalites) and short tandem repeats (strs)