ANSC 313 Lecture Notes - Lecture 15: Embryo Transfer, Controlled Ovarian Hyperstimulation, Foal
LECTURE 11!
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Biotechnology!
artificial insemination!•
embryo transfer!•
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Artificial insemination!
Cattle!•
about 95% of dairy cattle!◦
used "commonly" in purebred beef!◦
distributed frozen!◦
swine!•
used to a limited extent, mainly for disease control!◦
distributed fresh by overnight delivery!◦
gilts/ sows detected in heat and order is placed!◦
can't easily freeze pig semen ( very jelly like, does not freeze)!◦
horses!•
used by some breeds ( severe restrictions typical- i.e. within so many meters)!◦
collected & used within hours!◦
horses have venarial disease: to avoid it from spreading !◦
also kicking could damage the stallion while trying to mate!◦
poultry!•
all turkeys bred by A.I.!◦
used "fresh"!◦
dogs!•
limited use, mostly for problem mating cases!◦
can be frozen with limited success!◦
"super chilled"!◦
vaginal or surgical ( laporoscopy)!◦
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Embryo Transfer ( ET or MOET)!
established protocols for cattle!•
banned of some hormones will impact success/use!•
larger animals vaginal transfer, smaller ones by laporoscopy!•
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ET calves!
embryo transfer calves are simply full-siblings!•
quality of recipient matters!•
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ET Foals!
in spring 2004 first ET foals born from Calgary stampede`s bucking horses!•
more top name mares went through superovulation!•
method 1!•
12 mare- 17 ova- 6 transferred - 4 pregnancies!◦
didn't seem to work very well!•
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Sexing embryos!
cut portion off and use PCR with Y specific primers to sex!•
"damaged" embryos do not freeze well!•
therefore transfer fresh!•
