BIOL239 Lecture Notes - Repressor, Allolactose, Wild Type
13 Sep 2014
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Set 16: levels at which gene expression is regulated in prokaryotes. Some protein will bind to dna and stop transcription or enhance it: the role of rna polymerase in transcription. 35 and -10 (tata/pribnow box: the role of rna polymerase in transcription. 10 and -35 bind the sigma and then it leaves after initiation. But not all genes are being transcribed all the time: studies of e. coli metabolism. Used to determine fundamental principles of gene regulation in prokaryotes: The binding of regulatory proteins to dna target controls transcription. These regulatory proteins either inhibit or enhance transcription: lactose utilization in e. coli. Allolactose - modification of the bond which is broken by b-galactosidase. Watch this: http://www. pbs. org/wgbh/nova/genome/program_adv. html she talked about it for 15 minutes so it might be helpful to watch it! Also, there are multiple choice questions about it in podcast lecture 23. The presence of lactose induces expression of the genes required for lactose utilization.
