BIOL309 Lecture Notes - Lecture 21: Sanger Sequencing, Rna-Seq, Transcriptome
Document Summary
Northerns, in situ hybridization (less abundance but more localization), semi-quantitative. Rt-pcr: look at transcript abundance changes in specific genes. We don"t have an idea in mind so we are just going to profile or catalogue the transcripts present. This is called transcript profiling or monitoring the transcriptome. Then come up with the important transcripts that differ. Transcriptome is the complete mrna complement of a cell which is now expanded to include mirnas. Researcher looking for genes that may be important to a process. Using est abundance to monitor transcript abundance differences. Really are the precursor for rna seq because they were done when dna sequencing was very expensive. Sequenced with sanger sequencing (being done one at a time) Cdna libraries generated using mrna isolated from specific cells or organisms to identify different cdnas present, many at random clones from these libraries are partially sequenced; only one end of each clone is sequenced.
