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Lecture 2

BIOL442 - Lecture 2 - Phage.pdf

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University of Waterloo
BIOL 442
Christine Dupont

Set 2 PhageBacteriophageViruses of bacteriaEasy to study safe reproduce quickly1915 described by Twort accidentally discovered in bacteria contaminating smallpox vaccine virus culture1917 dHerelle a canadian at Pasteur Institute also observed a similar phenomenon and purused itCoined the name bacteriophage from the latin phagin to eatLater called the observed clearings plaquesLifestyle of bacteriphageLytic or virulent phage are those which can only lyse kill the bacterial hostEg T4Lysogenic or temperate phage phage that can either multiply via the lytic cycle or enter a quiescent state in the bacterial cell prophage eg Phage gammaNot all viruses lyse their host cellsLysogenic phase integrate DNA into bacterial chromosome and remain there for a period of time as a prophageAs a prophage little gene expression turned off by viral repressor proteinsThe virus is replicated with the bacterial chromosomeFilamentous phage an exception Eg M13 and f1Do not burst the cell exit individually as they are madeCapsid protein embeds in cell membraneHost cell survives if budding out is slowplaques appear as diffuse growthAssay for Lytic PhagePlaque AssayMeasurement ofof infectious particles virionsEach virion is a plaque forming unit pfuDilution series done to get countable number of plaques20200 or 300 depending on plaque size New Section 1 Page 1
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