BIOC 302 Lecture Notes - Lecture 16: Transferase, Purine, Chromatin
Document Summary
Q1: sigma protein by itself does not bind to bacterial promoter sites. Predict the efect of a mutaion enabling sigma to bind to the -10 region in absence of the other rna polymerase subunits ( 2, , ", It competes with the rna polymerase compeiive inhibiion. Q2: cordycepin inhibits rna synthesis explain its moa. If the oh is phosphorylated, it would be encorporated into the dna. Q3: rho binds to a rut site in the newly made rna. However, the rna polymerase may be far away down the template. Rho uses atp hydrolysis to move along (5"- 3") the rna, chasing the rna polymerase. When it catches the polymerase, the rna is pulled out of the dna rna-polymerase complex. You have a dna template that contains a promoter, a rut site 100 bp downstream of the promoter, and a rho-independent terminator 3 kb downstream of the promoter.
