BIOL 121 Lecture Notes - Lecture 2: Sister Chromatids, Chromatid, Centromere
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You find a way to attach either a red or green fluorescent dye to nucleotides. Double-stranded DNA molecules with both strands labeled red fluoresce red, those with both strands labeled green fluoresce green, and those with one strand labeled red and the other green fluoresce yellow. You grow human cells in the presence of âredâ nucleotides until both DNA strands of all chromosomes fluoresce red. You then allow one round of DNA replication in the presence of âgreenâ nucleotides. |
What pattern of fluorescence do you expect to see in the sister chromatids of each chromosome? |
a) one chromatid red, the other green
b) one chromatic green, the other yellow
c) both chromatids yellow
d) one chromatic red, the other yellow
Continuing your experiment with âredâ and âgreenâ labeled chromosomes, you allow cells with DNA labeled âredâ in both DNA strands to undergo two rounds of DNA replication in the presence of âgreenâ nucleotides. What pattern of fluorescence would you expect to see in the sister chromatids of each chromosome? |
a) one chromatid red, the other yellow
b) one chromatid red, the other green
c) one chromatid green, the other yellow
d) both chromatids yellow