BIOL 200 Lecture Notes - Lecture 19: Protein Targeting, Peptide, Signal Peptide
Document Summary
The endomembrane system: membrane compartments involved in processing and movement of proteins and membranes. Should be able to identify and describe the difference components of the endomembrane system. Er signal sequence and signal recognition particle (srp) direct ribosome to er membrane: srp binds to the exposed n-te(cid:396)(cid:373)i(cid:374)al er ig(cid:374)al e(cid:395)ue(cid:374)(cid:272)e (cid:894)aka (cid:862) ta(cid:396)t t(cid:396)a(cid:374)sfe(cid:396) Signal sequences for co-translational insertion of membrane proteins (all have a stretch of hydrophobic a. a. : start transfer sequence. N-terminal signal sequence (aka n-terminal start transfer sequence: cleaved off. Internal start transfer sequence: not cleaved off. Is a membrane crossing domain: stop transfer sequence, stops transfer of protein across membrane. Er targeting sequences are primarily short (14-18 aa) hydrophobic domains. The primary difference between these signal sequences is their location in the primary structure, not structure itself. If there is a n-terminal signal sequence (aka n-terminal start transfer sequence) the. Domain maps used to show predicted regions with specific function in the primary sequence of a protein.
