BIOL 341 Lecture Notes - Lecture 4: Uniprot, Microsoft Powerpoint, Genbank
Document Summary
Last week you and your classmates designed primer sets that would amplify the t7 promoter from pgem-t and introduce restriction sites at each end. This week you will also digest the pgfp-s12 vector in order to prepare it for the insertion of the. Calculate, mix, and dispense enzyme cocktails to minimize pipette error between samples. Conduct blast searches to find genes and proteins that are closely related to their own sequence of interest. Conduct sequence alignments to identify conserved regions within their sequence. Identify a protein"s family, domains, and sequences of interest, and use this information to suggest a structure and function. Find a 3d protein or domain structure and relate its structure to its function. Find scientific literature that could shed light on the function of their sequence of interest. Restriction analysis figure and results (individual; 3%) Plasmid dna is considered a level 1 biohazard. Discarded samples and contaminated plastics should be autoclaved.
