BIOL 344 Lecture Notes - Lecture 21: Ethidium Bromide, Southern Blot, Variable Number Tandem Repeat
Document Summary
Probability of inheriting a disease or genetic trait. Can sequence a single gene or a whole genome. Restricted enzymes will cut unmodified dna into pieces and recycle them to make their own dna. Recognizes 4, 6, or 8 nucleotide recognition site. Different version of the same r. e. and pick the one that is best for the job. Can visualize dna on agarose gels by ethidium bromide staining under uv light. Can determine size and relative abundance of dna molecules. Different molecules of similar size thus big smear. Used to detect and quantitate dna or rna. If detected a sequence and can find the dna of interest. Sequence allele and will detect difference in the level of dna. 80 percent of the 0. 1 difference is due to snps. Happen in regions that has no genetic consequences. Usually associated with diseases such as sickle-cell anemia. Can use a probe to detect rflp using southern blot.
