BCH 2333 Lecture 12: Lecture 14 - Types of enzymes
22 Mar 2017
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Try doing definition of how the enzyme works and make the equation from that. Usually no backwards formation of the ep from the e + p. Smallkcat is not the same as the k2. Shows efficiency of enzyme at converting substrate to product. Double reciprocal of the michaelis menten equation, factor out km. Extrapolate from the black dots which are experimental values. Cannot have negative substrate to extrapolate to x intercept. Specificity at same diffusion control rate = most efficient enzyme. Urease is not as great but still pretty good. Try to get kcat to reach diffusion control rate. Be able to know which enzyme by a equation or "story" Eg hemoglobin needs a porphyrin ring that can incorporate 2+ iron. Usually a small molecule that is the nonprotein section. Can change the enzyme to make more promiscuous. Shape and size dependent where the reaction occurs. Usually a slightly different environment than the cell within the binding site.
