BIO 3124 Lecture Notes - Lecture 22: Reverse Transcription Polymerase Chain Reaction, Microbiology, Epitope

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4 Apr 2018
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Establish and con rm the ethology of the disease. Track the progression of the infection in the patient: determine whether or not a treatment is effective. Purpose: identify infectious agent or follow progression of infection. Biochemical: identi cation based on metabolic characteristics (ex. oxygen requirements, carbon sources used, oxidative or fermentative metabolism, metabolic byproducts) When antibodies react with multiple epitopes on soluble antigens - formation of networks which generate an insoluble precipitate. Precipitation reactions can take place in solution or in gels, such as agar: aggregates become insoluble and generate precipitate. Phenomena of zones: precipitate formation is in uenced by the concentration of ag-ab. Too much antigen/too much antibody: no aggregates, no precipitation. Optional ratio: generate precipitate: zone of equivalence. December 1, 2016: optimal for formation of aggregates. Occurs with a valency of at least 2. Antigen test: determines whether or not an antigen is present. Coat latex beads with a given antibody - allows for a very high valency.

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