BIO206H5 Lecture Notes - Lecture 14: Kary Mullis, Phosphodiester Bond, Endothelium
Document Summary
Study note, bio206: all cells have a remarkable ability to synthesize dna with specific sequences. In pcr,we need to know something about sequence to be amplified: known dna or rna sequence, or, known homologous sequence, or, known protein sequence. In pcr, we need to have need to have: source of target dna (sequence, set of oligonucleotide primers, enzy(cid:373)e (cid:272)apa(cid:271)le of fo(cid:396)(cid:373)i(cid:374)g 3" 5" phosphodiester bonds (dna polymerase, thermally stable dna polymerase: Taq is the polymerase enzyme used in pcr originally isolated from thermus aquaticus: central to automated pcr is the discovery of a thermal stable dna polymerase. Taq polymerase from thermus aquaticus, a bacterium that lives in hot springs and hydrothermal vents. It converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Plasminogen is synthesized as a single chain, which is cleaved by plat into the two chain disulfide linked plasmin. This enzyme plays a role in cell migration and.
