BIO206H5 Lecture Notes - Flippase, Elongation Factor, Dna Replication
Bio 206, 2012 Final Exam 1
of
13
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UNIVERSITY OF TORONTO MISSISSAUGA
DECEMBER 2012 FINAL EXAMINATION
BI0206H5F
Introductory
Cell and
Molecular
Biology
George Espie
Duration - 2
hours
Aids:
Non-Programmable
Calculators
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Mark
Distribution: Questions 1 -60 & 82; 1 mark each =
61
Questions
61
-90; 2 marks each 58
Question
91
18
Question 92 32
Question
93
15
Question 94 10
Total
= 194
and
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Bio 206, 2012 Final Exam 2
of
13
On the scantron sheet, indicate whether the following statements are; TRUE (A) or FALSE (B).
1 Cholesterol increases the fluidity
of
a phospholipid bilayer by decreasing hydrophobic interactions.
2 Triacylglycerol is a phospholipid.
3 The nuclear lamina is a protein-meshwork that provides structural support for the nuclear envelope.
4 Each
of
the numerous sigma factors found in a bacterial cell recognizes a consensus sequence
at
-35
bp which is unique to that sigma factor. All genes and operons regulated by that sigma factor will
have that specific
-35
consensus sequence in the region
5'
to the start site
of
transcription.
5 During DNA transcription a 9 base pair DNA-RNA hybrid is formed within the Wsubunit
of
RNA
polymerase.
6 Bidirectional transport
of
proteins across the nuclear envelope requires the participation
of
the HSP70
chaperone protein.
7 Eukaryotic RNA polymerase I is responsible for the transcription
of
pre-tRNA genes while RNA
polymerase III transcribes pre-rRNA genes.
8 The TIM23 complex facilitates translocation
of
targeted proteins into the matrix
of
the
mitochondrion.
9 Amphipathic a-helices contribute to the formation
of
membrane channels.
10
The anti-Shine-Dalgarno sequence is located at the
5'
end
of
the
16S
rRNA and has the consensus
sequence, AAUGGU.
11
The 23S rRNA and the 28S rRNA carry out equivalent functions in 70S and
80S
ribosomes,
respectively.
12
The proteins CstF and CSPF participate
in
the alternative splicing oflmRNA.
13
Centromeres are located at the ends oflinear, eukaryotic chromosomes.
14
Protein chaperones assist in the folding and unfolding
of
other proteins and are essential for import
of
proteins into the chloroplast and mitochondrion.
15
The
3'
splice site at an intronlexon junction is characterized by the presence
of
a pyrimidine rich
region
15
-
45
bases upstream
of
a conserved nucleotide sequence
5'
AG 3'.
16
The protein factor EF -G is a receptor protein that identifies proteins targeted to the Golgi complex.
17
The first co-transcriptional complex to arrive at the CTD
of
RNA PolII is the capping complex.
18
During the elongation phase
of
RNA transcription, the continued association
of
general transcription
factor TFIID is absolutely essential.
Bio 206, 2012 Final Exam 3
of
13
19
DNA polymerase is capable
of
proof
reading while RNA polymerase and PolyA polymerase are not.
20 DNA replication commences in an ordered fashion at sites separated by 15,000 nucleotide base pairs.
21
The protein eIF-4A is required for processing Okazaki fragments.
22 Rab-GTP is required for vesicular docking and membrane fusion.
23
COPII -coated vesicles shuttle proteins between the plasma membrane to the Golgi complex.
24 A nucleoside
is
composed
of
a pentose sugar and a nitrogenous base while a nucleotide is composed
of
a pentose sugar, a nitrogenous base and a phosphate group.
25
Pex 5 is a receptor protein that binds the SKL targeting sequence.
26 In a globular protein the amino · acid lysine
is
most likely to be found within the core
of
the structure.
27
3'
to
5'
exonuclease activity is an enzymatic feature common
to
both DNA polymerase and RNA
polymerase.
28 The nuclear lamina is functionally equivalent to the cortex
of
the plasma membrane.
29 NBS's are short amino acid sequences rich in hydrophobic amino acids that are used
to
target proteins
for export from the nucleus.
30 RAN-GTP is a protein that adds the NLS sequence to proteins destined for the nucleus.
For
each
of
the following molecules, macromolecules
or
subcellular particles listed in questions
31
to
60
below indicate the processes (A to G) it participates in . Choose the ONE
BEST
answer for each
question.
The
same response may be used
more
than
once. Place
your
answer
(A to G) on the scantron
form. NOTE: Pay attention to the question
numbers
when filling the scantron form.
A.
DNA replication / synthesis
B.
Transcription
C. RNA processing and splicing
D. Translation
E.
Membrane / cortex structure / cytoskeleton -synthesis and lor assembly
F.
Chromosome/chromatin structure
G.
Protein targeting/import/export
31
flippase 32 Pribnow box
33
aminoacyl tRNA 34 snRNP U2
35
RF2 36 enhancer sequences
37 scaffold attachment region 38 spectrin
39 cAMP-CAP complex 40 SAM complex
