BIO315H5 Lecture Notes - Lecture 20: Coiled Coil, Cyclin-Dependent Kinase 1, Sister Chromatids

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20 Nov 2017
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Replication only occurs once in the cell cycle and it is regulated through regulating initiation. It occurs in two steps: it occurs in late mitosis/early g1. The licensing of replication origins and the activation of origins must occur. The orc (origin recognition complex) is used where it is already bound to the origin of replication at numerous origins and is scattered in every chromosome. S-cdk phosphorylates initiator proteins which nucleate assembly of a large protein complexes. These complexes activate helicases that are also activated by phosphorylation by ddk and recruit dna synthesis machinery needed for the initiation of replication. Explain figure 17. 18: control of the initiation of dna replication. The replication origin is bound by the orc throughout the cell cycle. In early g1, cdc6 associates with the orc, and these proteins bind the dna helicase, which contains six closely related subunits called mcm proteins. The helicase also associates with a protein called cdt1.

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