BIO315H5 Lecture Notes - Lecture 19: Transmembrane Protein, Symporter, Connexin
Document Summary
The chart below shows actin filament dynamics in the presence of atp. Referring to the chart and the equations given below, answer the following questions: Indicate the exact concentration where steady-state treadmilling occurs. Yellow line (plus): y = 2x - 16. Steady state treadmilling is the defined as the condition where the rate of growth at one end is (cid:271)ala(cid:374)(cid:272)ed (cid:271)y the rate of loss at the other e(cid:374)d su(cid:272)h as fila(cid:373)e(cid:374)t le(cid:374)gth does(cid:374)"t (cid:272)ha(cid:374)ge. The equations are related to each other when you have steady state treadmilling: Yy + yred = 0: the initial subunit concentration was 16 and the total volume of the reaction mixture was 1 liter. You dilute the solution by adding an extra 1 liter of water. Both ends are growing, dilute to 1/2 --> becomes 8 rather than 16: rather than atp, you use atpys, a non-hydrolyzable form of atp, in the initial reaction mixture.
