BIO315H5 Lecture Notes - Lecture 11: Ubiquitin Ligase, Cytosol, Endosome
Document Summary
If it gets trafficked to the extracellular space, it tends to get oxidized. N-acetylglucoseamines, high energy phosphate bond is here: add mannose using gdp mannose, once this step is done, the entire intermediate gets flipped, the other sugars are added, not with the help of udp and gdp intermediates but with. It now faces then lumen of the er mannose or glucose that are conjugated to dolichol (mannose phosphate dolichol) If the protein has maintained the properly folded state, it will exit the er. If it is normally folded, it is released into the er, if not, another enzyme glucosyltransferase adds the glucose back on so that calnexin can be bound. If there is lot of misfolded proteins, it binds to ire1. This receptor oligomerizes, autophosphorylate (it has kinase activity) Once the receptor is activated, the cytosolic face has enzyme activity called endoribonucleases. Endoribonucleases are enzymes that cleave nucleic acids. Endoribonucleases gets activated and causes splicing of mrna.
