BIO372H5 Lecture Notes - Lecture 8: Integrase, Dna Replication, Tyrosine
Document Summary
Notes - site-specific recombination and transposition of dna. Cells and viruses use conservative site-specific recombination (cssr) for biological functions. 1) many phages insert their dna into the host chromosome (infection) 3) maintain the structural integrity of circular dna molecules during dna replication, homologous recombination, and cell division. 1) serine recombinases: introduce dsb in dna. A serine residue within the protein"s active site. 5" p-serine bond with dna after dna break. Phosphate-serine bond that links the serine recombinase protein to the dna. 2) tyrosine recombinases: break and rejoin one pair of dna strand at a time. A tyrosine residue within the protein"s active site. A tyrosine in the protein"s" catalytic site. 3" p-tyrosine bond with dna after dna break. Serine break both strands of dna vs. tyrosine only breaks one pair of. 1) a pair of recombinase-binding sites : positioned symmetrically, recombinase recognition dna sequences. In between the recombinase recognition sequences is the crossover region.
