BIO206H5 Lecture Notes - Lecture 9: Small Nuclear Rna, Intron, Exon
Document Summary
Now we"re talking about how the primary transcript is processed. A single nucleotide difference in the splce cycle can cause tremendous damage to the. Rna molecule, this is why exon splicing is so importan. This is an example of where we have both protein catalysts and rna catalysts working together: assembly of 5 separate slurps. What you can see in pic is that there"s as ingle mismatch, that pushes that rna molecule. Gonna be a catalytic site for the splicing reactions: typically we think of rna molecules as being single stranded. Like a molecular beacon: at 5 prim eend u see recruiting of u1. Once everyone is in position, this provides the platfrom to which the u4,6,5 will bind: so if u1 and u2 aren"t there, u4,6,5 don"t bind to intron. The binding site for them is constructed by the interaction of u1 and u2 with the intron.
