CHM361H5 Lecture Notes - Lecture 8: Amide, Hydrolysis, Tetrahedral Carbonyl Addition Compound
Document Summary
Rotation possible about n-ca (amino acid 2) and ca-c(amino acid 1) No rotation about c-n (as a partial double bond) Trans conformation or the carbonyl o and amide h. Analysis of primary structure -2 general approaches. Provides info regarding composition but does not give you primary structure. Acid hydrolysis of polypeptide (with 6n hcl at 110 celsius for. Every single peptide bond will be hydrolyzed amino. Mechanism of acid hydrolysis of peptide bonds (memorize this) The ones they elute first (not a good stick) Only aromatics (f, y, w amino acids) For free amino acids they will be derivatized with phenyl isothiocyanate. Every amino acid has the same absorption profile because tagged with this benzene group. Polar, and charged amino acids derivatives will come of first. No asn (n) or gln (q) left after hydrolysis. There is an amide bond in the side chain in. These amide bonds will be hydrolyzed and you will get two products.
