PSY355H5 Lecture Notes - Lecture 10: Telepathy, Transfection, Pharmaceutical Industry
Document Summary
Rats and mice mainly used for esc for double selection process bec these esc are not available for other species. So with tale it can cut out or damage any gene. There is much more elegant method than talen system- the crispr/cas9 system: clustered redundant interspaced palindromic repeat. Nuc sequences that have a specific seq, and these seq are repeated. The regions in between these (the ones that are not repeats) are the interesting parts. These in between regions are actually viral seq. But the repeated ones are bacterial sequences. But ethical issues: something like if removing huntington gene is not ethical issue, but if someone says that they want to have child with higher iq. With crispr you do not have control over the mutations- all we know is that there is roughly going to be a mutation here. Likely of recombination with inserted seq is high because they are very homologous and complementary.
