BIOC13H3 Lecture Notes - Lecture 8: Citric Acid Cycle, Acetyl-Coa, Glyoxylic Acid
Document Summary
Lecture 8: citric acid and glyoxylate cycles (continued: isocitrate dehydrogenase, goal of cac is to oxidize acetyl coa, the first oxidation reaction, isocitrate (secondary alcohol) oxalosuccinate (ketone) alpha. Nadh and fadh2, which will be released upon passage of electrons to o2 via. In an anaplerotic pathway, this is a problem: bypass these 2 oxidation steps, 2 unique enzymes. 1930s: dehydrogenase, cytochrome series, oxidation, wieland discovered a dehydrogenase that oxidizes. Nadh: warburg found an oxidase could reduce oxygen, keilin"s cytochromes that link these together, the heme group in cytochromes is what carries the electrons, the respiratory chain was water-insoluble, means it was membrane-associated. If you know the concentrations of adp and phosphate supplied, you know how much. Respiration: uncoupling agents uncouple respiration from atp synthesis, stimulate respiration but inhibit atp synthesis, dnp (dinitrophenol), fccp uncoupling agents, decrease in atp synthesis, respiration increases (nadh oxidation occurring, no atp synthesis)
