CHEM 212 Lecture Notes - Lecture 8: Analyte, Dont, Analytical Chemistry

• School: University of Victoria
• Department: Chemistry
• Course: CHEM 212
• Professor: Hamilton M.Coreen

Document Summary

1: internal standard--> f, standard addition has two methods, constant final volume /standard addition to multiple solutions (intercept for result, varying nal volume or standard addition to a single solution. *single point by either a or b is a ratio. Varying final volume technique-std addition to a single solution: Plot on x-axis: volume of standard after dilution. Cs= standard solution concentration vo=initial volume of sample. Signal(sample+spike)*(vf/vo) vf= volume of sample +spike intercept at y=0 gives - [unknown sample] Very labour intensive to carry out addition experiment --> don"t do this. Usually do single point std addition: constant nal volume, varying nal volume. Clues for choosing which method is being used: nothing added to sample in case 1 --> external standard similar compound added in case 2--> internal standard analyte added in case 3 --> standard addition--> ratio. Concentration of analyte plus standard in nal solution. Ss+s=k([a]+[s]) signal from initial solution signal from nal solution.