BIOL 2131 Lecture Notes - Lecture 9: Nanopore Sequencing, Nanopore, Dna Microarray
14 Mar 2016
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Document Summary
Dna is being fed through a single pore that is connected to an electric field. With a single pore, you get a quarter of a million bases per second: fastest sequencing out there possible (and that is just with a single nanopore) Unique thing is that the dna is never degraded: in all other techniques discussed, it is cut up into millions of pieces, you can re-sequence the same dna over again for accuracy purposes. Relatively cheap because materials used to make it are relatively inexpensive: no chemicals other than the enzyme that brings in the dna molecule to link to the pore. Idea is that dna sequencing is going to be portable: will be able to do it anywhere. Advantages of nanopore sequencing: fast (250 000 bp/sec)/pore, dna is not degraded can re-sequence, cheap. Future advancements: disposable sequencers (, want to ramp up high through-put so that you can get 8000 pores/device.
