BIOL 3070 Lecture Notes - Lecture 5: Lysosome, Caseous Necrosis, Gram Staining

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coppersheep743

8 Dec 2017

School

University of Windsor

Department

Biological Sciences

Course

BIOL 3070

Professor

tanyanoel

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Document Summary

The respiratory system can be divided into the upper and the lower respiratory. We"ll typically discuss respiratory pathogens by the location of. Now, please note that in the textbook, and for our. 0:17 purposes, we are considering the larynx to be in the lower respiratory tract. 0:21 fact it could be included in both upper and lower, so part of the larynx, the part. 0:27 above the vocal cords, is usually considered to be the upper respiratory. 0:31 tract, while the part below the vocal cords would be considered lower. 0:35 our purposes we"ll just consider it to be in the lower respiratory tract. 0:43 are some general points here about respiratory infections particularly when. 0:46 we compare those of the upper respiratory tract and those of the lower. There are a wide range of pathogens that can cause respiratory. 0:55 diseases and we"ll be looking at a number of them but not in much depth, with.

Related Questions

The epiglottis acts as a

voice box.

filter for microorganisms and debris.

cover over the larynx.

producer of mucus.

ANSWER:

(Level 1, Objective 1)

Collectively, the nose, oral cavity, pharynx, epiglottis, and larynx make up the

nasopharynx.

oropharynx.

laryngopharynx.

upper respiratory system.

ANSWER:

(Level 1, Objective 1)

The cilia present on cells of the lower respiratory system function by:

trapping microorganisms and foreign debris.

propelling microorganisms and particles out.

preventing colonization by potential pathogens.

all of the above.

ANSWER:

(Level 1, Objective 2)

The most common causative agent of a swollen and painful oropharynx is

Streptococcus pyogenes.

Arcanobacterium haemolyticum.

Corynebacterium diphtheriae.

Rhinovirus.

ANSWER:

(Level 1, Objective 3)

Infections associated with cystic fibrosis are due to inflammation of the:

bronchi.

bronchioles.

lungs.

alveoli.

ANSWER:

(Level 1, Objective 3)

A physician sends a nasal washing for viral culture. This specimen is

unacceptable because it is contaminated with upper respiratory flora.

acceptable as long as it contains epithelial cells.

unacceptable if it is for the detection of influenza or respiratory syncytial viruses.

acceptable if it has a thick consistency and is yellow or green in color.

ANSWER:

(Level 2, Objective 4)

A patient is unable to produce adequate sputum for routine, acid-fast, and fungal cultures. The physician may attempt to obtain a/an

induced sputum.

transtracheal aspirate.

bronchoalveolar lavage.

pleural fluid.

ANSWER:

(Level 1, Objective 4)

9. Two throat swabs are received from a 6-year-old female with orders for a rapid screen for group A Streptococcus. One swab is tested using the group A Streptococcus rapid screen, and it is negative. The second swab is used to inoculate

a. sheep blood agar.

b. chocolate agar.

c. no culture is necessary.

d. a and b.

ANSWER:

(Level 1, Objective 6)

Which of the following disease states requires the addition of selective and/or differential medium to ensure recovery of potential pathogens from the respiratory system?

Pertussis

Cystic fibrosis

Laryngitis

a and b

all of the above

ANSWER:

(Level 2, Objective 6)

11. A throat culture provides the following results:

Moderate growth of alpha-hemolytic, catalase-negative, optochin-susceptible, gram-positive diplococci

Heavy growth of alpha-hemolytic, catalase-negative, optochin-resistant, gram-positive cocci

Heavy growth of gamma-hemolytic, oxidase-positive, glucose- and maltoseÂ-positive, gramÂ diplococci

You would report

moderate growth of Streptococcus pneumoniae and heavy growth of normal upper respiratory flora.

heavy growth of Streptococcus pneumoniae and heavy growth of normal upper respiratory flora.

heavy growth of Neisseria meningitidis and heavy growth of normal upper respiratory flora.

heavy growth of normal upper respiratory flora.

ANSWER:

(Level 3, Objective 7)

Yeast colonies in a sputum culture are considered significant when they

are present.

comprise 25% of the organisms present.

comprise 50% of the organisms present.

comprise 75% of the organisms present.

ANSWER:

(Level 1, Objective 7)

13. An 8-year-old male presents with a red and sore throat. The rapid group A Streptococcus screen is negative, and a sheep blood agar is inoculated. At 24 hours, there are no beta-hemolytic colonies present and moderate growth of normal upper respiratory flora. At 48 hours, the same culture results are present. Which of the following statements does not explain these results?

a. His sore throat is due to Haemophilus influenzae, and it will not grow on blood agar.

b. The culture was taken very early in the infection.

c. The patient received treatment prior to specimen collection.

d. The causative agent is not bacterial.

ANSWER:

(Level 2, Objective 8)

17. A quantitative bronchial alveolar lavage culture requires:

1. sheep blood agar.

2. chocolate agar.

3. 0.01 mL pipette.

4. 0.001 mL pipette.

a. 1 and 3

b. 1, 3, and 4

c. 2, 3, and 4

d. 2 and 4

ANSWER:

(Level 1, Objective 9)

18. A bronchial washing is plated to a BCYE. This agar should be examined under a laminar flow hood due to the potential for recovery of Legionella pneumophila and

a. Bordetella pertussis.

Francisella tularensis.

mould.

Pneumocystis jiroveci.

ANSWER:

(Level 2, Objective 9)

20. A physician submits a sputum with a culture request for Legionella. Select the growth medium that should be used to cultivate this organism.

a. 7H10

b. TCBS

c. BCSA

d. BCYE

ANSWER:

(Level 1, Objective 6)

coppertoad665

The following CASE REPORT is given:

This 3 1/2-year-old boy was born to nonconsanguineous parents from Chihuahua, Mexico after an uncomplicated pregnancy and delivery. A brother had died at 3 months of age of a "liver problem" after an unexplained coma. The parents and three siblings are well.

At 3 months of age the boy was admitted in coma to a community hospital and suffered a cardiac arrest. Examination revealed hepatomegaly and cardiomegaly and a serum glucose of 15 mg/del (normal, 60-100 mg/dl). The patient eventually recovered and the hepatomegaly resolved. At 6 months of age he developed congestive heart failure after an upper respiratory tract infection and was brought o the medical center of the University of California, Los Angeles. Hepatomegaly and hypotonia were noted on admission. ON day 3 he became lethargic and developed generalized seizure activity and cardiac arrest, but he was successfully resuscitated. Laboratory studies at the time of the arrest revealed a blood glucose level of 15 mg/dl without associated acidosis or ketosis; mild elevation of serum aspartate aminotransferase (SGOT) (337 I.U./liter; normal, 6-36 I.U./liter) and alanine aminotransferase (SGPT) (179 I.U./liter; normal, 10-45 I.U./liter); and hyperammonemia (300 ug/dl; normal <69 ug/dl). Delayed milestones (developmental quotient 66; normal, 100), proximal-muscle weakness, and growth retardation (weight and height below the 3rd percentile) were noted after recovery. Metabolic studies showed normal glucose, galactose, and fructose tolerance, and a normal 10-hour fasting blood glucose, with no increase in lactate, pyruvate, or ketone bodies. The electroencephalogram, brain scan, and chromosomal studies were unremarkable. Also normal were the plasma levels of electrolytes, calcium, phosphorus, magnesium, bilirubin, thyroxine, thyroid-stimulating hormone, and growth hormone. Results of total serum protein determination, serum electrophoresis, cerebrospinal fluid studies, and studies of immune function were also normal. Between acute episodes, serum levels of glucose, ammonia, SGOT, SGPT, and creatine phosphokinase were all normal.

The patient was again admitted with cardiorespiratory arrest after upper respiratory tract infections at the ages of 20, 24, and 33 months. The episodes were associated with liver enlargement, elevations of transaminases to >2000 I.U./liter, and of creatine phosphokinase from 1500 to 33,000 I.U./liter. Maintenance glucose requirements varied from normal (3 mg/kg of body weight per minute) to slightly elevated (5-7 mg/kg/min).

A muscle biopsy specimen contained large amounts of neutral lipids. Liver biopsy also showed severe but nonspecific fatty changes. Abnormal mitochondrial structure, many electron-dense lysosome-like bodies, and dense, laminate, rounded lipofuscin-like particles were evident.

A thirty-two hour fasting study was performed. During the fasting period the patient had no nausea, cramps, or pigmenturia. However, at thirty-two hours he suddenly had a cardiorespiratory arrest characterized by an absence of cardiac electrical activity. He was successfully resuscitated and intravenously glucose was administered.

At the start of the fast his blood glucose level was 92 mg/dl, but by twenty-four hours it had fallen to 66 mg/dl. Plasma triglyceride levels rose from 66 to 126 mg/dl and free fatty acids increased from 0.1 to 2.0 mEq/liter. Serum SGOT rose from 36 to 1450 I.U./liter, with no increase in creatine phosphokinase or aldolase. Ammonia increased from 40 to 134 ug/dl. The most significant finding, however, was the lack of production of measurable ketone bodies.

During the 11th admission, the diagnosis of carnitine deficiency was considered because of fatty changes in the liver and the lack of production of ketone bodies after twenty-four hours of fasting. At that time the patient's height and weight were normal but his developmental quotient was 40, and he had a variety of neuromuscular abnormalities. A computerized axial tomography (CAT) scan of the brain revealed marked enlargement of both lateral ventricles and of the sulci between the cerebral gyri.

QUESTION: What is the cause of hyperammonemia in this patient and would this be a symptom of myopathic carntine deficiency too?

pinkwasp329

44.Charlotte touches a doorknob after an individual with MRSA touched the doorknob. Immediately after touching the knob, Charlotte scratches her leg and transmits some MRSA cells into a small wound.The doorknob is functioning as a(n) a. etiologic agent. c. mode of transmission. b. reservoir. d. host. 44.A man infected with a viral respiratory infection sneezes and releases tiny droplets containing viral particles. Two minutes later, a woman breathes in some of these tiny droplets and becomes infected by the same viral infection. The movement of this virus from one individual to another is best described as the a. etiologic agent. c. mode of transmission. b. reservoir. d. host. 45. Although sporadic outbreaks of measles occur in the United States, the disease is considered nonendemic. What differentiates measles from endemic diseases, such as chickenpox? a. Endemic diseases exhibit higher numbers of cases than a sporadic outbreak. b. Diseases that are not endemic lack a reservoir in the area. c. Endemic diseases do not exhibit sporadic outbreaks. d. Diseases that are not endemic remain in reservoirs without infecting humans. 46. Which of the following would indicate efforts to control a disease are working? a. The disease becomes endemic. b. The disease prevalence increases. c. The disease incidence decreases. d. Outbreaks of the disease only occur every other year. 47. Which of the following statements describes one of the three steps of surveillance used for infectious diseases? a. Patients with infectious diseases are required to be seen by a physician at the CDC. b. Physicians are required to submit all patient records electronically to the CDC for review. c. Patient charts are reviewed by an infection control practitioner when an infectious disease is suspected. d. Incidence data is collected in all cases of bacterial infections within the United States. 48. Which of the following lists correctly identifies nosocomial infections from most common to least common? a. urinary tract, pneumonia, septicemia b. pneumonia, urinary tract, surgical wounds c. septicemia, surgical wounds, diarrhea d. diarrhea, urinary tract, pneumonia 49. Mary, an elderly woman with an indwelling catheter, is admitted to a local hospital for treatment of pneumonia. Three days after her admission, Maryâs husband receives a call from the hospital. He is informed that Mary has a nosocomial infection. Which of the following statements is true? a. The nosocomial infection referred to is pneumonia. b. Mary has most likely developed a urinary tract infection. c. Of all hospital patients, 25â30% develop nosocomial infections. d. Maryâs nosocomial infection could have been prevented with improved ventilation. 50. A nurse working in a large hospital harbors MRSA as part of the normal microbiota in his nose. He is most likely the source of a MRSA outbreak if a. infected patients have all had been cared for by this nurse and the isolates are identical. b. the patients are all elderly and the isolates of MRSA are not genetically identical. c. all of the infected patients were seen by the same physician and half of the patients were cared for by this nurse. d. all of the infected patients are on the same floor of the hospital and all have a history of heart problems. 51. Which of the following is NOT an example of aseptic technique? a. use of antiseptic on skin prior to insertion of an IV line b. use of latex gloves during the insertion of an indwelling catheter c. disinfection of doorknobs and other inert surfaces d. use of trash can liners in all patient rooms 52. An individual case of diphtheria, a notifiable infectious disease, in a patient in Florida is confirmed by laboratory testing. This patientâs physician would be required to a. send a letter to the WHO in Geneva, Switzerland. b. contact the CDC in Atlanta, Georgia. c. treat the patient regardless of health insurance status. d. quarantine the patient for eight weeks. 53. Fifteen individuals within one province in China are diagnosed with SARS. Epidemiologists will most likely a. quarantine the entire province immediately. b. contact the CDC in the United States to see if any cases were reported in the United States. c. wait to see if more cases of SARS are reported. d. try to identify the index case. 54. How does vaccinating wild rodents or poultry influence human health? a. Vaccinating wild rodents or poultry has no effect on human health. b. Vaccinating wild rodents or poultry reduces transmission of zoonotic diseases to humans. c. Vaccinating wild rodents or poultry reduces the migration of microbes into temperate regions. d. Vaccinating wild rodents or poultry increases the emergence of drug-resistant bacteria. 55. Which of the following scenarios would help meet the goal of the One Health Initiative? a. vaccinating poultry against avian influenza to prevent influenza from being transmitted to humans b. taking steps to reduce carbon dioxide emissions to reduce the effects of climate change c. providing sanitation after flooding events to prevent gastrointestinal infections in humans d. increasing compliance during antibiotic treatment of tuberculosis patients to prevent emergence of drug-resistant strains of tuberculosis 56. If humans continue to develop rapid transportation systems into more parts of the world, we should expect to see a. pandemics tracing the path of the transportation systems. b. an increase in detection of blood-borne pathogens. c. a decrease in human encroachment into tropical rain forests. d. an increase in the spread of transmissible spongiform encephalopathies. 57. How has the AIDS pandemic influenced reemerging diseases? a. AIDS has increased the number of immunocompromised individuals who are susceptible to diseases that were previously declining in developed countries. b. AIDS has led to an increase in the number of disease vectors throughout the world. c. AIDS has increased the prevalence of protozoal and parasitic infections that were declining in underdeveloped countries. d. AIDS has caused evolution to occur in diseases that were close to being eradicated in all countries. 58. Which of the following is likely to occur as a result of climate change? a. Insect vectors will migrate from tropical regions to once-temperate regions. b. Gastrointestinal infections will decrease in tropical regions. c. Microbes currently found in temperate regions will move to tropical regions. d. Extreme weather will decrease and diseases will become less seasonal. 59. What features would allow a virus to be used effectively as a bioweapon? a. a high infectious dose c. person-to-person transmission b. transmission via an insect vector d. quick pathogen detection systems

BIOL 3070 Lecture Notes - Lecture 5: Lysosome, Caseous Necrosis, Gram Staining

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