Biochemistry 2280A Lecture Notes - Lecture 20: Conformational Change, Start Codon, Peptide

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Bonnie Mac Lachlan

Document Summary

Ea(cid:272)h (cid:272)odo(cid:374) is (cid:1007) (cid:271)ases (cid:271)e(cid:272)ause there are (cid:1006)0 aa therefore (cid:374)eed (cid:1006)0 (cid:272)odo(cid:374)s some codons specify for the same aa (64 codons, yet only 20 aas) Increases the chance of a functional protein in the case of a single base mutation. Types of mutations: missense: 1 base changes and aa changes, silent: 1 base changes but aa remains the same, frame shift: insertion/deletion of a base (very problematic, nonsense: 1 base changes and creates premature stop codon trna. Bring the aa to growing polypeptide chain. Structure is due to internal base pairing. Has non-conventional bases (put in after transcription) Two key ss regions: 3" acceptor site: where aa is attached (cca phe, anticodon: base pairs with codon. Catalyze protein synthesis (translation) really big molecules (have both rna and protein) ~ 4. 2 mda. 3 binding sites for trna (only 2 occupied at any one time: a site: binds aminoacyl-trna, p site: binds peptidyl-trna, e site: site which trna exits.