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Biochemistry 2280A Lecture Notes - Lecture 3: Tata Box, 5.8S Ribosomal Rna, 28S Ribosomal Rna

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spectral
11 May 2020
School
Western University
Department
Biochemistry
Course
Biochemistry 2280A
Professor
Mclachlin

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Document Summary

Rna polymerases 1,2 and 3 carry out transcription; Polymerase i transcribe 28s, 18s and 5. 8s ribosomal rna genes. Polymerase ii transcribes all protein coding genes and few non-protein coding genes** Polymerase iii transcribe trna, 5s ribosomal genes and other small rna genes. Genes that are expressed in all cell types (ubiquitous) are required for universal cell functions such as: dna repair, protein synthesis, cell survival, nucleic acid synthesis and metabolic pathways. The amount of gene expression in the cells can vary depending on nutrient availability, rates of tissue growth and cell growth. Differentially expressed protein coding genes makes all cells different, important during development. e. g. contractile proteins are present in muscle cells, but not brain cells. A protein domain: a separate part of the tertiary structure that can develop and function without the main protein. Dna binding domain recognises 3 b. p. of double stranded dna. Required to stabilise its structure - wrap around the double helix.

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The function of tRNA is to

Select one:

a. provide a site for polypeptide synthesis

b. transport amino acids to the ribosome

c. transcribe DNA

d. transform DNA

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The lipid bi-layer is

Select one:

a. hydrophilic

b. hydrophobic

c. hydrophilic and hydrophobic

d. depends on the surrounding medium

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Which of the following statements is FALSE?

Select one:

a. carbohydrates are generally hydrophilic molecules.

b. living things use carbohydrates for structure and for energy storage.

c. carbohydrates are composed principally of carbon, hydrogen, and oxygen.

d. none of the above – they are all true

Question 4

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Which of the following terms correctly describes a compound featuring more than one double bond?

Select one:

a. saturated

b. polyunsaturated

c. monounsaturated

d. disaturated

Question 5

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Eukaryotic mRNA transcripts are protected from modification by

Select one:

a. 5' caps

b. 5' poly-A caps

c. 3' caps

d. 5'-3' poly tails

Question 6

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The methods of membrane transport that don't require protein channels or carriers are

Select one:

a. osmosis

b. diffusion

c. phagocytosis

d. exocytosis

e. all of the above

Question 7

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Complete the following sentences: "Primary active transport is dependent on __________. Secondary active transport is dependent on __________ created by __________.

Select one:

a. channel proteins, electrochemical gradient, carrier proteins

b. electrochemical gradient, ATP, mitochondria

c. electrochemical gradient, ATP, primary active transport

d. ATP, electrochemical gradient, primary active transport

e. carrier proteins, electrochemical gradient, channel protein

Question 8

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Which of the following is a reduced compound?

Select one:

a. CO2

b. O2

c. N2

d. NAD +

e. NADH

Question 9

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Gel electrophoresis separates DNA fragments according to their:

Select one:

a. base sequence

b. size

c. percentage of labelled nucleotides

d. electrical charge

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G0 is:

Select one:

a. part of interphase

b. the phase of the cell cycle where DNA replication occurs

c. the phase of the cell cycle where two identical daughter cells are created

d. a special "resting" phase in which cells are in a non cycling state

e. a special phase where the number of chromosomes in a parent cell is reduced by half such that each daughter cell contains only one member of a pair of homologous chromosomes

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At which pH will the concentration of hydrogen ions be equal to the concentration of hydroxide ions?

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a. pH 1

b. pH 5

c. pH 7

d. pH 9

e. pH 14

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Which of the following enzymes has proofreading activity?

Select one:

a. Ligase

b. Helicase

c. Topoisomerase

d. DNA polymerase

e. Peptidyl transferase

Question 13

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The process of _______________ cuts introns from the primary transcript and the final "processed" mRNA is produced.

Select one:

a. RNA cleaving

b. RNA translocation

c. RNA elongation

d. RNA splicing

Question 14

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Transcription is initiated when RNA polymerase binds to

Select one:

a. a promoter

b. an initiator

c. a transciptor

d. a codon

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Complete the following sentences: "__________ connect the extracellular matrix with microfilaments. __________ connect intermediate filaments from adjacent cells."

Select one:

a. Cadherins, Integrins

b. Connexins, Cadherins

c. Integrins, Cadherins

d. Integrins, Connexins

e. Connexins, Integrins

Question 16

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Which of the following regions of RNAs are translated?

Select one:

a. 5' UTR

b. exons

c. introns

d. 3' UTR

e. poly-A

Question 17

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Control of gene expression in eukaryotic cells occurs at which level(s)?

Select one:

a. only the transcriptional level

b. epigenetic and transcriptional levels

c. epigenetic, transcriptional, and translational levels

d. epigenetic, transcriptional, posttranscriptional, translational, and posttranslational levels

Question 18

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Cellular organelles containing hydrolytic enzymes are called:

Select one:

a. peroxisomes

b. lysosomes

c. ribosomes

d. mesosomes

Question 19

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______ are chemicals or combinations of chemicals that take up excess hydrogen ions or hydroxide ions in order to keep the pH constant.

Select one:

a. acids

b. bases

c. salts

d. buffers

e. carbohydrates

Question 20

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Which of the following proteins appear in some stages of the cell cycle and are degraded at other stages?

Select one:

a. kinases

b. cyclins

c. nucleotides

d. phosphatases

e. none of the above

Question 21

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Which of the following correctly matches an organelle with its function?

Select one:

a. mitochondrion photosynthesis

b. nucleus cellular respiration

c. ribosome manufacture of lipids

d. lysosome movement

e. central vacuole storage

Question 22

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What is the sequence corresponding to the following electropherogram obtained by Sanger sequencing (green: A; red:T; black: G; blue: C)?

Select one:

a. CCGTTATGAAC

b. GGCTTATGAAG

c. CAACTATTGCC

d. GGCAATAGTTG

e. CCGTTATCAAC

Question 23

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Which of the following statement is true

Select one:

a. the two DNA strands are parallel and complementary

b. the two DNA strands are anti parallel and non-complementary

c. the two DNA strands are anti-parallel and complementary

d. none of these

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The energy-requiring process by which small molecules are joined to form larger molecules is specifically called

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a. metabolism

b. anabolism

c. catabolism

d. enzymatic reactions

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When DNA replication starts

Select one:

a. the phosphodiester bonds between the adjacent nucleotides break

b. the bonds between the nitrogen base and deoxyribose sugar break

c. the leading strand produces Okazaki fragments

d. the hydrogen bonds between the nucleotides of two strand break

Question 26

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A(n)_______ is the movement of a segment of a chromosome from one chromosome to another nonhomologous chromosome

Select one:

a. inversion

b. translocation

c. deletion

d. duplication

Question 27

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Complete the following sentences: "Replication forks from the same origin of replication extend __________. __________ DNA strand is used as template for the leading strand at one fork and for the lagging strand at the second fork. New DNA is synthesized from 5' to 3' on the __________."

Select one:

a. unidirectionally, A different, both the leading and lagging strands

b. bidirectionally, The same, leading strand

c. bidirectionally, The same, lagging strand

d. unidirectionally, A different, leading strand

e. bidirectionally, The same, both the leading and lagging strands

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Manufacturing recombinant DNA molecules involves cutting a gene from its normal location, inserting it into a circular piece of DNA from a bacterial cell, and then transferring the circle of DNA to cells of another species. Which of the below describe the circular piece of DNA?

Select one:

a. restriction enzyme

b. plasmid

c. bacteriophage

d. transposon

Question 29

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Complete the following chemical equation to make it balanced: glycerol + ___ ? triacylglycerol + ___

Select one:

a. 3 fatty acid, 3 H2O

b. fatty acid, H2O

c. 3 OH-, 3 H+

d. 3 H2O, 3 fatty acid

e. fatty acid, 3 H2O

Question 30

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Which structure is found in plant cells but not animal cells? Check all that apply.

Select one or more:

a. Cell wall

b. Chloroplast

c. Plastid

d. Central vacuole

e. Lysosomes