Biochemistry 2280A Lecture Notes - Lecture 16: Fluorescent Tag, Genetic Engineering, Brca2
11 May 2020
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Target identification have no suspect genes, shortlisting the target genes: Combine equal amounts of the sample and add them to the chip: when rewashed the chip will show three colours, one for sample a, b and both, those only present in one sample are then investigated individually. To find out which proteins regulate a protein of interest you look for physical interactions between the two. Yeast-2-hybrid screen: performed in yeast a simple eukaryotic system, so comparable to humans, you start with the known protein of interest (poi), looking for other proteins that interact with the poi. Applications in medicine: diagnostic detection of pathogenic organisms (pcr) or endogenous mutations, predictive brca1 and brca2 genes help control cell division in breast and ovarian tissues. Point mutations can lead to elimination of restriction enzymes sites, when this happens there will be a change in the sites of dna fragments produced. This change becomes that marker for the mutation, and therefore the disease.
