Biochemistry 2280A Lecture Notes - Lecture 23: Escherichia Coli, Recognition Sequence, Conformational Change
25 Dec 2014
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The rna step provides an amplification which contributes to differential gene expression, ex. W-->x-->y-->z: often the first step is most highly regulated as it saves energy; ex. -> z step, the cell would consume 2atp that makes the w--> x and x---> y. Transcription is the 1st step in the expression of any gene and is often highly regulated! Downstream from that is +2 and upstream is -1 (there is no 0) 10 sequence--> 10 bases pairs upstream of +1. One approach taken was to ask if one or more common sequence elements is found in different e. coli promoters. In this example, none of the elements are the same as the consensus sequence o ( it doesn"t have to exist--> it is a statistical thing) Normal gene gives 100 units of activity and mutated give 5 units of. What is the consensus sequence for the dna element shown above--> The rna polymerase with the dna is called a closed complex.
