Biology 2382B Lecture Notes - Lecture 2: Antonie Van Leeuwenhoek, Dichroic Filter, Fluorescence Microscope
9 Feb 2018
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First to observe living protozoa and bacteria, achieved a magnification of 200x. Resolution: ability to distinguish between two very closely positioned objects as separate entities. Conventional light microscope usually cannot resolve objects/cellular features lower than 0. 2 nm: we can"t see viruses. Amplitude of the wave is proportional to the intensity of light. Lightbulb not used, instead very powerful light source (mercury light, etc : damaging to the naked eye because it contains uv light. Dichroic mirror reflects light down into objective lens then strike the specimen on the stage, then excite the fluorochromes on the specimen, then different red light passes through dichroic mirror and is then processed through the emission filter. Fluorescent microscope"s light covers a broader wavelength (300-700nm) and is more intense. Main function: reflect excitation wavelength and allow emitted light to pass through. Face contrast: uses a less intense light (400-700 nm range)
