Biology 2581B Lecture Notes - Lecture 5: Intron, Microsporidia, Noncoding Dna
12 Feb 2017
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Document Summary
The length of one set of those instructions. A set of genetic instructions within a biological compartment. Add up all the different genomes to find genome size. Mass of dna has direct correlation with the length of the dna. Haploid human nuclear genome has a mass of 3pg with a length of 3 billion bp. Human mitochondrial genome is about 0. 000016 pg with a length of 16 kbp. Sequence then assemble and do a word count. Find overlaps and create contigs then get this size. Add up all the different length contigs and try to estimate size of genome. Next generation sequencing is not always good for big genomes. Works for dna that are small in length (approx. Use reagents that when poured on dna, stick to it and fluoresce. Send that stained cell through imaging (flow cytometry) and estimate size. Can also be imaged through microscopy, intensity of staining is related to genome size.
