Class Notes (837,061)
Canada (510,044)
Biology (6,817)
Lecture

Lecture 2

2 Pages
84 Views
Unlock Document

Department
Biology
Course
Biology 3466B
Professor
Yolanda Morbey
Semester
Fall

Description
Handout 2 • Large effect mutations o Marfan syndrome (large people, heart problems and other defects in body) • Mutation rate = number of mutations/locus/generation or number of mutations/genome/generation o Methods to compare mutation rates  Population survey • Large population to get good accuracy  Direct methods using molecular data • Observing mutation in action across generation and counting how many mutation rates happen • Cant do in humans but can in some model organisms • Survey: HIV infection pathway o Hard because we don’t have lots of mutation data across generations o See handout for diagram for how HIV works o Correceptor CCR5 • CCR5 genotyping o Steps:  Extract DNA  Amplify gene using polymerase chain reaction (PCR) – gets lots of copies of gene of interest  Cut DNA sequences with restriction enzyme  Run fragments on electrophoresis gel o Survey the distribution of CCR5-∆32  Individuals with deletion are more resistant to HIV1 Survey: Genetic diversity of CF gene • Survey: genetic diversity of CF gene o People with CF have disfunctional gene and are unable to fight off bacteria and as a result have difficulty breathing and absorbing nutrients by the intestine. Not expected to live beyond their 30s o There are over 500 mutations that can cause CF that all occur at this gene o Every where there is a blue line is a spot where you can nuclear get CF DNA o 15000 individuals, 30000 alleles. Graph shows the survey of the gene o Mutations occur all the way along the length of the gene but most common in the binding region • May be surveys to see mutation in characteristic or you may have to test the genome • Direct method: in C. elegans o Mutation accumulation line(MAL) Direct method: in C. elegans  Allows them to live in an environment with all the things they need to survive. Very
More Less

Related notes for Biology 3466B

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit