Biology 1002B Lecture Notes - Lecture 5: Heat Shock Protein, Protein Folding, Peptide
21 Feb 2017
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Document Summary
Process that takes info in dna for protein coding gene and uses it to synthesize a protein. 4 things that can dictate how much protein is synthesized. Transcription- takes info in gene and converting it to mrna: amount of protein created depends on rate of transcription. Mrna degradation: mrna is unstable= degrade, lots of degradation vs. little amount of degradation. Measure gene expression: not easy to measure transcription/translation of a certain protein, measure protein abundance, transcript abundance- how much of a certain mrna mrna stability. Total rna- large bands= ribosomal (90% is ribosomal) Rna is intrinsically unstable: degree of instability can be altered, rna with intricate folding are more resistant to breaking down (ribosomal is less prone to breaking down than messenger because it is more folded) Allows temporal regulation of gene expression: wa(cid:455) of (cid:862)swit(cid:272)hi(cid:374)g off(cid:863) ge(cid:374)e e(cid:454)p(cid:396)essio(cid:374) (cid:271)(cid:455) letti(cid:374)g (cid:373)rna de(cid:272)a(cid:455, modulate things much more finely, genes that code for housekeeping proteins tend to have longer lived transcripts.
