Biology 1002B Lecture Notes - Lecture 21: Dna Microarray, Hybridization Probe, Rna-Seq
16 Aug 2017
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Document Summary
Gene function regulation of gene expression & products genes encode & role of products in the function of the organism. High degree of similarity between sequence of a candidate gene of unknown function & sequence of gene of known function sequences are homologous (evolved from same gene in a common ancestor) Sequences conserved significantly because they code for proteins with similar functions. Information about the function of a gene in one well-studied species very often applies to the homologous genes in another. Gene knockout: replace normal gene with defective gene that cannot express a functional protein. Defective gene lacks orf that encodes the gene"s protein product (genetically engineered deletion: haploid organism one copy of gene to knock out, diploid organism both copies of gene must be knocked out. On a genomic scale, experimental manipulations can be done to knock out each gene systematically one by one. The phenotypic consequences of zero expression of each gene can then be ascertained.
