Biology 1002B Lecture Notes - Lecture 9: Expression Vector, Microinjection, Fluorescent Tag

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How channelrhodopsin can control action potentials in neurons: action potentials can be controlled by channelrhodopsin from chlamy, a neuron in the brain of the mouse and we go to chlam and take chlamy. Possible sources of genes (ie. channelrhodopsin) for use in making transgenic organisms: from where they live in the nuclei of organisms, genes can be created by reverse transcription of mrna to make dna (extract. If you wanted to cu the chalmy genome into small pieces would you use the. 4cutter or 6cutter: 4cutter because the sequences will be more common, and more frequent will lead to smaller pieces. Basic mechanism for creation of cdna: take mrna and reverse transcribe it to make double stranded dna. Ways in which cdna versions of genes would be different than genomic versions: cdna would not have a promoter because making cdna from transcript. Role of plasmid (expression) vectors in genetic engineering.

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