Biology 1002B Lecture Notes - Lecture 18: Channelrhodopsin, Optogenetics, Halorhodopsin
30 Mar 2016
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How might hpv cause cancer without causing mutations: could make mirna that stops expression of tumour suppressor. Optogenetics provides control of specific neurons at specific times in behaving animals. Positive ions move inside and then depolarize the membrane potential. Chlamy"s channelrhodopsin stuck inside mouse expressed in neurons of a mouse. When the flashes of blue light activated: changes the conformation of the channelrhodopsin and positive ions flow through causing action potential: control of nerve firing. Need chlamy"s channelrhodopsin: where to get the gene: get it from chlamy"s nucleus and chop out the gene we"re after. Pull out chlamy"s nuclear dna: sources of genes: obvs the nuclei then cut them out, also mrna, use restriction enzymes: 4-cutters and 6-cutters, run out on gels and stuff. Go into genome of chlamy and get the channelrhodopsin gene specifically then paste later. We can also get genes from: mrna, reverse transcribe to make the rna into double stranded dna cdna (complementary.
