Biology 1201A Lecture Notes - Lecture 6: Electrophoresis, Sticky And Blunt Ends, Helicase
2 Oct 2016
School
Department
Course
Professor
Document Summary
It attaches to it and injects viral dna into the bacteria. The bacteria has to figure out a way to destroy viral dna without destroying its own bacterial dna. It does this by methylating its own dna (by the enzyme methylase) as it is synthesized, so the bacteria recognizes non-methylated dna as foreign. Restriction enzymes are floating around in the cell, and if unmethylated / viral dna enters, the. Res will go destroy viral dna: restriction enzymes work by recognizing palindromic dna sequences. If such a sequence is methylated, it will leave it alone. If it"s not, it will cleave the strand in two, leaving two sticky ends: ex: strand gaattc is cleaved to g aattc. Start with a bacterial plasmid dna, and cut with ecor1. Cut an insulin gene with ecor1, as well. This leaves 2 sticky ends on both the plasmid and the insulin gene that are complementary.
