Biology 1202B Lecture Notes - Covalent Bond, Nucleoside Triphosphate, Hydroxy Group

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Dna replication: dna is replicated semi-conservativley meselson and stahl used two different isotopes for nitrogen ( n15 & n14) and added e. coli to the medium. The lighter band is higher up and the darker band lower down. Isolates dna see how the dna settles. If the clamp was not there, polymerase would polymerize and then disassociate making the process very slow. Starts off with an origin of replication various binding protiens (cascade of molecules will start to process) Helicase unbinds the helix of dna energy dependant (hydrolyzed atp) Single stranded binding proteins proteins that bind to the individual strand to prevent it from becoming double stranded again. Topoisomerase nixes the backbone allowing it unwind releasing/relieving tension on the helixes. Rna primase recognizes origin of replication and lays down rna primer (3") attaches to clamp and starts putting bases down. No telomerase = shorter life span but less cancer.

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