Biology 2290F/G Lecture Notes - Lecture 9: Pericentriolar Material, Intermediate Filament, Axoneme

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Intricate network of protein filaments that extend throughout the cytoplasm. Microfilaments 7-9nm, microtubules 25nm, intermediate filaments 10nm. Named according to their size: smallest is microfilaments (actin), intermediate filaments, largest ones are microtubules (tubulin) These are the different proteins that make up the cytoskeleton: microfilaments, microtubules, intermediate filaments. Movement achieved by cell migration, movement within the cell. Organelle/protein trafficking, cilia/flagella, mitosis/cytokinesis, muscle contraction, cell adhesion, cell migration, extravasation. Hollow tubes, 25nm diameter, up to 100s of micrometres long. Largest component of the cytoskeleton in terms of diameter (25nm: these proteins can polymerize (monomers form dimers, forming microtubules) Microtubules make up cilia and flagella, which stick out of the cell. Different isoforms of tubulin; main component of microtubules are alpha and beta tubulin: both about 55kda each (very similar to each other) When cell translates alpha and beta monomer, they instantly form a dimer, which is the basic building block. If cell is building microtubules it"s using dimers.

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