a. For the serial dilution experiment you take 10µL of an E. coli culture and dilute it in 990µL LB. You do a total of 4 dilutions and plate 100µL of each dilution on a separate LA plate. You see 29 colonies on the second to last plate. What is the cell concentration of the original bacterial culture?
b. How many colonies would you see on the last plate?
c. For the plaque assay, your T4 bacteriophage titer is 6.8x109 pfu/mL. You do six 1:10 dilutions of the virus, putting 10µL of each dilution in a tube of top agar with 100µL of E. coli. After pouring the contents of the top agar onto an LA plate, you incubate overnight. How many plaques would you expect to see on the final plate?
