BIOL 2040 Lecture Notes - Functional Group, Micelle, Column Chromatography
24 Aug 2020
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Jan 21 biol2020: how many of the 20 amino acid, what is the highest energy. Protein charge and ph: the overall charge of a peotein depends oin. Ph of the solution: protins with high pl are more basic. Bind more tighly to cation exchange resin: protiens with high pl are more acdic. Bind more loosly to cation exchange resin. Native protein conformation: single poyltpeptide (img, a dimer : homodimer, oligomer / multisubunit, dimer two, homodimer two identical, hetero two different. 2: amino acid, dipeptide, tripeptide, = peptide, polypeptide. Questions: what is its sequence and composition, what is its three-dimensional. Separation by charge ; ion exchange: common ion exchange resins. Separation by size ; size exclusion: big proteins come out first, small slower than bigger one. Protein visualization after sds page: coomassire dye, siver, functional group, wetern blor to membrane after electophage, crude lyste, flow thru, lon exchange, affinity, pure protein (single bend of the gell correspond to purified protein)
