BIO 4307 Lecture Notes - Lecture 8: Differential Centrifugation, Centrifugal Force, Proteome
Document Summary
Introduction: proteome is derived from proteins expressed by the genome. The genome provides a list of gene products that could be present but only a small number will be expressed. The proteome tells us what proteins are functional present and unlike the genome the proteome is not a fixed characteristic. It varies with cell type, environmental conditions, or developmental stage. Commonly done by blending, mincing or grinding etc: after the homogenate is formed this liquid mixture is centrifuged by two methods: Thus, organelles that are larger will be at the lower band of the test tube than smaller organelles. After each purification method, an assay or a test that is used to see if purification is working is done: why might a biochemist do this, the assay is based on some identifying characteristic of the target protein. Besides this information, a biochemist has to also know the amount of total protein present in the mixture being assayed.
