BIOLOGY 1AL Lecture Notes - Lecture 11: Yeast Extract, Luciferase, Eyepiece
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Ex: at 10x magnification, there are 10 ticks; each tick represents 10 micrometers. If you increase it 20x, then each tick represents 20 micrometers. Single bacteria away from any other spot to generate a colony over time. Bacteria don"t always divide geometrically because of nutrient limitations and toxins that build. Complex yeast extract, peptone you don"t know the concentration of the different molecules in there. Defined where you know the concentration of the molecules. Selective high salt concentration media with antibiotics only bacteria that are resistant to antibiotics. Differential put something in media where you can distinguish between other types of bacteria. Luciferase will generate blue light and uses energy. As you increase density of bacteria, that molecule builds up and as it builds up, it feeds back the synthesis of its gene, and produces more enzyme and signaling molecule. In addition to this, you also increase the mrna concentration for luciferase, and that"s why you get bioluminescence.
